Caspr Cas12 Turbo 1.0
COVID-19 Detection Kit
CASPR Lyo-CRISPR
SARS-CoV-2 assay
Our assays harness CASPR’s
proprietary CRISPR-Cas12 complex,
in a novel and convenient lyophilized
format, designed to detect
SARS-CoV-2 RNA sequences in
clinical samples with sensitivity and
specificity comparable to
gold-standard technologies.
Features
Enhanced
collateral activity
Active in a wide range
of temperatures
Optimal activity at
room temperature
Detects picomolar
concentrations
Questions we get, often.
Product Overview
Description
Wild type recombinant enzyme obtained from a gene identified in metagenomic samples, with site specific DNA endonuclease activity. This Class 2/Type V enzyme has been assigned to the Cas12p subfamily.
Guide RNA
45 nucleotides long-guide RNA consisting of a loop (22 nucleotides) plus a region (23 nucleotides) fully complementary to the target DNA sequence.
PAM sequence
TTTV
Applications
Pathogen detection
Mutation detection
Optimal Activity
Conditions
From 25 to 50 °C
Incubation time: 30 min
Readout
Fluorescent reporter in solution, lateral flow (reactive strips)
Product Specifications
Chemical and
Physical Properties
Molecular Weight: 150.3 kDa
Format: Liquid
Purity: 99% (evaluated by SDS-PAGE)
Reaction
Conditions
Activity Buffer: 50mM NaCl, 10mM Tris-HCl pH 7.9, 10mM MgCl2, 100 µg/ml BSA
Temperature: 25º-50º C
Suggested Working concentration: 1-2 μM. (diluted in storage buffer without glycerol)
Time: 30 min
Storage
and Shipping
Storage Conditions: -20°C at stock concentration
Stock Concentration: 4 μM.
Storage Buffer: Tris-HCl 50 mM (pH 8), NaCl 200 mM, MgCl2 20 mM, DTT 1 mM, 50% Glycerol
Shipping: Dry Ice
Performance data
Enhanced collateral activity comparable to other Cas enzymes in the market
FIG. 1 Trans-cleavage
activity using
single-stranded
DNA reporters.
A. Endpoint cleavage 5, 10, and 20 minutes
B. Time course cleavage.
The specificity of trans-cleavage activity was tested using customized ssDNA 5’6-FAM TTATTATT-3IABkFQ3’ as reporter. The results showed a comparable cleavage between the enzymes with . Detection assay was performed in a fluorescence plate reader after a 20 min incubation at 25°C using complexes to a final concentration of 75 nM Cas : 75 nM sgRNA.
Active in a wide range of temperatures
FIG 2. Activity of CASPR
Cas12 Turbo 1.0 at
different temperatures
(25-50°C).
The efficiency of trans-cleavage activity at different temperatures was tested using customized ssDNA 5’6-FAM TTATTATT-3IABkFQ3’ as a reporter. The results showed that CASPR Cas12 Turbo 1.0 is able to cleave with similar efficiency the ssDNA reporter in a wide range from room temperature to 50°C. Detection assay was performed in a fluorescence plate reader after a 20 min incubation at 25, 30, 37 and 50°C using CASPR Cas12 Turbo 1.0 complexes to a final concentration of 37.5 nM Cas : 37.5 nM sgRNA
